Composite
Part:BBa_K1053004
Designed by: Ippei Sakamoto Group: iGEM13_Tokyo-NoKoGen (2013-09-10)
PBAD- TR-HHR(RBS)-GFPuv-DT
This part consists of a gene that is coding GFPuv and taRNA responsive HHR which has a stem loop including RBS. HHR has self-cleavage activity, and RBS will be exposed when self-cleavage will occur. However, its activity is suppressed when taRNA(taR12) hybridizes to HHR. Therefore, in the presence of taRNA, the expression of GFPuv is lower than in the absence of taRNA.
A: pSB1A3-PBAD-taR12-DT and pSB3C5-PBAD-TR(12)-HHR(RBS)-GFPuv-DT
B: pSB1A3-ΔRFP and pSB3C5-PBAD-TR(12)-HHR(RBS)-GFPuv-DT
C: pSB1A3-ΔRFP and pSB3C5-ΔRFP
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 1856
Illegal XhoI site found at 1757 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1271
Illegal SapI site found at 961
[edit]
Categories
Parameters
None |